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Figure 2 | Molecular Pain

Figure 2

From: Mutation I136V alters electrophysiological properties of the NaV1.7 channel in a family with onset of erythromelalgia in the second decade

Figure 2

I136V mutation alters deactivation and steady-state slow-inactivation. A, Time constants of channel deactivation. Cells were held at -100 mV and tail currents were generated by a brief 0.5-ms depolarization to -20 mV followed by a series of repolarizations ranging from -100 to -40 mV to elicit tail currents (insert a). I136 mutant channels (n = 19) show significantly slower deactivation than NaV1.7R channels (n = 17) at all testing potentials. Insert b shows the representative tail currents from two HEK293 cells expressing NaV1.7R (solid line) or I136V mutant channels (dotted line). B, I136V mutation significantly enhances steady-state slow-inactivation. Steady-state slow-inactivation was examined by a series of prepulses (30 s) from -130 to +10 mV followed by 100-ms return pulse to -120 mV, then a 20-ms test pulse to -10 mV.

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