DRG manifest both GFP-labeled neurons and glia. (A-B) GFP-labelling in L4 DRGs of control mice on sham sugery and intact sides, respectively. (C-D) GFP-labelling in L4 DRGs of nerve injured mice on injured and contralateral sides, respectively. Higher density of glia cells in injured DRG (C) contrasts that of intact DRG (D). The GFP-labeled neuron in (D) indicated by arrow is shown in the inset at a higher magnification, arrowheads point to the satellite cells in close apposition to the neuron. (E-F) Confocal laser scanning microscopic images of injured and intact L4 DRGs, respectively. Arrowheads and arrow point to the Schwann cells and satellite cell, respectively. (G) The density of glial cells was significantly higher in injured L4 DRG than in its contralateral counterpart (p < 0.05, n = 3). "Contr." and "CpNL" indicate control and nerve injured mice, respectively. (H) Number of different-sized L4 DRG neurons in intact side of control mice (bin size = 100). (I) the size of L3/L4 DRG neurons with strong labeling showed no change after nerve injury (p > 0.05, n = 3). The area is expressed as Mean ± SEM (μm2/per neuron). "Contr." and "CpNL" indicate control and nerve injured mice, respectively. Bar = 75 μm in A-B; 50 μm in C-D, 20 μm in E-F.