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Figure 4 | Molecular Pain

Figure 4

From: Oxytocin-induced antinociception in the spinal cord is mediated by a subpopulation of glutamatergic neurons in lamina I-II which amplify GABAergic inhibition

Figure 4

c-Fos expressing cells in the superficial layers of the dorsal horn after electrical stimulation of the ipsilateral PVN. A: Labeled c-Fos positive nuclei were localized in the most superficial layers i.e. lamina I and outer lamina II (IIo). B: Double immunoperoxidase labeling with an antibody against NpOT showed NpOT positive fibers (brown; arrowheads on inset) in the vicinity of c-Fos expressing nuclei (blue grey; arrows on inset). C: Double immunofluorescence labeling with an antibody against glutamic acid-decarboxylase (GAD) showed c-Fos positive nuclei (red cy3 fluorescence) in lamina I and IIo exhibiting a low GAD-immunoreactivity (green FITC fluorescence) whereas lamina IIi was strongly labeled by GAD antibody. D, E, F: In double immunofluorescence, c-Fos positive nuclei (arrowheads) never exhibited GAD labeled cytoplasm, despite the proximity in lamina IIo of GAD-immunoreactive neurons (arrows). A: 25 μm thick section; B: 1.5 μm semi-thin section; C-F: 30 μm thick sections. Sale bars: 100 μm in A and C; 10 μm in B and D-F. Individual colour channels on merged images were adjusted in C-F.

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Molecular Pain

ISSN: 1744-8069

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