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Figure 4 | Molecular Pain

Figure 4

From: TRPM8 mechanism of autonomic nerve response to cold in respiratory airway

Figure 4

TRPM8 receptor-mediated excitation of airway VG neurons. a. Micrographs show a DiI-labeled VG neuron (top) and subsequent patched-clamp recording (bottom). b. A sample trace shows a whole-cell inward current evoked from the neuron by a 5°C of temperature drop within 20 s. c. Inward current evoked by 100 μM menthol in the same neuron as in (b). d. Pooled results from experiments shown in b&c. Holding potential was -70 mV in b-d. e. I-V relationship of menthol-evoked currents from a VG neuron. f. I-V relationship of menthol-evoked currents from an acutely dissociated DRG neuron. g. a comparison of menthol-evoked currents in VG neurons (n = 7) and DRG neurons (n = 21). Cells were held at -70 mV. h&i. Action potentials elicited by 100 μM menthol (h) and cold (i) in the same VG neuron. All recordings were made from menthol-sensitive neurons pre-identified using Ca2+-imaging technique. Menthol was applied at 100 μM for 20 s. Cold stimulation was achieved by a 20-s application of cold bath solution which yielded a 5°C temperature drop from 26 to 21°C.

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