Figure 1

FLIPR-based library screen of 15d-PGJ2 activation of TRPA1. A. Fluorescence increases of TRPA1-expressing or mock transfected controls in response to a 50 μM 15d-PGJ2 stimulus. Traces represent average increases of cells from a 96-well plate. B. Fluorescence increases shown as minigraphs within 48 wells of TRPA1-expressing cells in response to increasing concentrations of 15d-PGJ2. All concentrations are in μM. 100 μM ATP and buffer were delivered as controls (the strain of CHO cell used exhibits endogenous ATP response and was used to ensure cell viability after compound addition).