Figure 2
From: Modulation of chloride homeostasis by inflammatory mediators in dorsal root ganglion neurons
(A) Two-photon fluorescence lifetime imaging microscopy (2P-FLIM) of intact DRGs loaded with the chloride indicator dye MQAE. The colour code indicates the fluorescence lifetime (τ) which is inversely proportional to the Cl- concentration. Warm colours represent high intracellular Cl- concentration (small τ values). (B) Quantitative evaluation of 2P-FLIM data show a highly significant increase of the inverse lifetime (LT = 1/τ) two hours after the start of treament with inflammatory mediators. Mean LT values were determined in control DRGs (LTctr) and in contralateral test DRGs (LTtest), and the percent increase of LT is depicted as ΔLT = (LTtest-LTctr)/LTctr·100 [%] for each treatment time. Indicated significance levels are p ≤ 0.05 (*) or p ≤ 0.01 (**).