Figure 3
From: Modulation of chloride homeostasis by inflammatory mediators in dorsal root ganglion neurons
Altered expression of Cl- transport proteins during treatment with inflammatory mediators. (A) Results from the segment-pair protocol where the mean immunofluorescence from each test DRG (FItest) is related to that of its contralateral control DRG (FIctr). The change of fluorescence intensity ΔFI = (FItest-FIctr)/FIctr·100 [%] is depicted 1 hr, 2 hr, and 3 hr of treatment, respectively. The data show that 3 hr after the start of the treatment the expression of the Na+, K+, 2Cl- cotransporter NKCC1 is enhanced while that of the K+, Cl- cotransporter KCC2 is reduced. (B) A similar result was obtained in experiments where control DRGs and test DRGs were not obtained from the same vertebral segment, but were randomly combined. Indicated significance levels are p ≤ 0.01 (**) and p ≤ 0.001 (***). Numbers in parentheses indicate cells evaluated.