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Figure 1 | Molecular Pain

Figure 1

From: Proteomic profiling of neuromas reveals alterations in protein composition and local protein synthesis in hyper-excitable nerves

Figure 1

Strategy for comparing neuroma and nerve cell protein composition. (A) Dye-labelling strategy for 2D-DIGE experiment profiling of mouse neuroma and control saphenous nerves. Control-1 to Control-3 indicates triplicate control samples. N3d-1 to N3d-3 indicates triplicate 3 day neuroma samples. N1w-1 to N1w-3 indicates triplicate 1 week neuroma samples. N2w-1 to N2w-4 indicates quadruplicate 2 week neuroma samples. N3w-1 to N3w-3 indicates triplicate 3 week neuroma samples. Additional preparatory gels were run with a higher loading of control sample. (B) Representative example of a 2D-DIGE analytical gel: 50 μg of control sample was labelled with NHS-Cy3 shown in green, 50 μg of neuroma sample was labelled with NHS-Cy5 shown in red and 50 μg of pooled internal standard (equal amounts of control and neuroma sample) was labelled with NHS-Cy2 shown in blue. The three Cy-dye labelled samples co-separate on one gel. Images were acquired on a Typhoon 9400 mutli-wavelength scanner. Around 1800 protein spots were detected on each gel. Below is a merged image of the three Cy-dye labelled images.

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Molecular Pain

ISSN: 1744-8069

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