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Table 3 Locally synthesized proteins identified after S35 methionine labelling of neuromas and control saphenous nerves.

From: Proteomic profiling of neuromas reveals alterations in protein composition and local protein synthesis in hyper-excitable nerves

Spot no

Protein name

Accession no.

MOWSE score

Est. mass

Est. pI

% Cov

53

Annexin A5

gi|74142393

172

35787

4.83

56

39

Vimentin

gi|2078001

127

51590

4.96

52

45

Actin, gamma 1 pro-peptide

gi|4501887

86

42108

5.31

33

11

Moesin

gi|70778915

68

67839

6.22

35

60

Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide, isoform C (14-3-3 zeta)

gi|148676868

61

29240

4.71

35

41

Vimentin

gi|31982755

57

53712

5.06

22

15

Protein disulfide isomerase associated 3

gi|112293264

52

57099

5.88

28

92

mTERF domain-containing protein 1, mitochondrial precursor

gi|81901619

46

47385

6.18

16

25

Unnamed protein product

gi|74210074

45

30222

9.34

26

38

Heat shock protein 1

gi|31981679

45

61089

5.67

37

12

Protein phosphatase 2, regulatory subunit B, delta isoform

gi|148685891

42

33815

6.18

9

50

Creatine kinase

gi|10946574

41

42971

5.4

24

47

Neurofilament-L

gi|200038

36

61542

4.63

11

62

Unnamed protein product

gi|26352624

40

15849

11.26

7

69

ORM1-like protein 1

gi|81174966

41

17401

9.56

37

73

S100 calcium binding protein G

gi|14789635

41

9150

4.69

57

8

Annexin A6

gi|31981302

42

76294

5.34

22

  1. All proteins were identified by LC-ESI-MS/MS peptide sequencing. NCBI gene identifier (gi|) number, MOWSE score from Mascot searches using LC-MS/MS data, estimated molecular mass and pI, and the percentage coverage of the protein sequence where matching peptides were found are given for each protein.