Figure 2
From: The role of cation-dependent chloride transporters in neuropathic pain following spinal cord injury
Early changes in expression of NKCC1 and KCC2 proteins in epicenter spinal cord tissues following SCI. A. NKCC1 protein expression in epicenter spinal cord tissues at 2, 7, and 14 days post-SCI. Sham (S) samples were acquired from animals subject to laminectomies without subsequent spinal cord contusion. Top panel, the blot was probed with anti-NKCC1 antibody (1:4000) and anti-βIII-tubulin antibody (1:5000). Lower panel, Densitometric analysis of the ratio of NKCC1/βIII-tubulin band intensity. Data were mean ± SD, n = 6. * p < 0.05 vs. Sham. B. Decrease in expression of KCC2 following SCI. Top panel, the blots were probed with anti-KCC2 antibody (1:500) and anti-βIII-tubulin monoclonal antibody (1:5000). Lower panel, summary of densitometry data for the ratio of KCC2/βIII-tubulin. Data were mean ± SD, n = 3. * p < 0.05 vs. S.