Figure 8

Immunohistochemical detection and immunofluorescence double labelling of CXCR2 in acutely dissociated DRG neurons and in DRG sections from rat. A: Double staining of the dissociated DRG cells with anti-CXCR2 and anti-NeuN showing that high CXCR2-expressing cells are neuronal cells, and that the more modest staining levels observed occur in almost all DRG neurons. B: Neurons with intense CXCR2 staining are all IB4-negative. C: CXCR2 staining in DRG sections showing expression on plasma membrane of most neurons, of small, medium and large diameters, with high levels expression in some smaller neurons. CXCR2 is also expressed in the nuclear membrane in some cells (arrows), as can be seen more clearly in the higher magnification view in D. E: Negative control. DRG sections incubated with primary antibody preabsorbed with 30 fold excess of antigen did not show any immunoreactivity. Scale bar = 50 μm. F. Western blot of protein isolated from DRG neurons, using the same antibody (1:300) as a probe. Left panel: standard. Right panel: CXCR2.