Table 1 Primers used for qPCR experiments:
Name | Rat gene symbol | Rat gene ID | Rat mRNA reference | Primers Forward, reverse | Predicted size (bp) | Position in mRNA |
|---|---|---|---|---|---|---|
Nav 1.1 | Scn1a | 81574 | NM_030875.1 | atccgagtccgaagatagca | 137 | 1817..1953 |
| Â | Â | Â | Â | gtctcggggaaaacagtgag | Â | Â |
Nav 1.2 | Scn2a1 | 24766 | NM_012647.1 | cggagagttcgtcagtagcc | 229 | 1661..1889 |
| Â | Â | Â | Â | aagagagactggtgcggaga | Â | Â |
Nav 1.3 | Scn3a | 81574 | NM_013119.1 | aacttggtgccatcaaatcc | 182 | 4513..4334 |
| Â | Â | Â | Â | cagattcacacccatgatgc | Â | Â |
Nav 1.4 | Scn4a | 25722 | NM_013178.1 | caccgctcttctctgcttct | 282 | 23..304 |
| Â | Â | Â | Â | gtgggtgtcctggtcttgtt | Â | Â |
Nav 1.5 | Scn5a | 25665 | NM_013125.1 | ttctagctcgaggcttctgc | 132 | 727..858 |
| Â | Â | Â | Â | tgcgtaaggctgagacattg | Â | Â |
Nav 1.6 | Scn8a | 29710 | NM_019266.2 | tacagtggctacagcggcta | 274 | 2008..2281 |
| Â | Â | Â | Â | tgtttgtgaccacgctcatt | Â | Â |
Nav 1.7 | Scn9a | 78956 | NM_133289 | gacagcttcttccagaggtgataata | 215 | 1922..2024 |
| Â | Â | Â | Â | ccatggtggacatttttgtct | Â | Â |
Nav 1.8 | Scn10a | 29571 | XM_001078257 | cacggatgacaacaggtcac | 151 | 1610..1760 |
| Â | Â | Â | Â | gatcccgtcaggaaatgaga | Â | Â |
Nav 1.9 | Scn11a | 29701 | NM_019265 | ggcagccaagtcaatctttc | 233 | 679..911 |
| Â | Â | Â | Â | gggccacagttgtgcttaat | Â | Â |
HPRT | Hprt | 24465 | NM_012583 | gcagactttgctttccttgg | 278 | 495..772 |
| Â | Â | Â | Â | tactggccacatcaacagga | Â | Â |