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Table 2 Membrane properties of isolated control and GRO/KC treated neurons in whole cell current clamp experiments

From: The chemokine CXCL1/growth related oncogene increases sodium currents and neuronal excitability in small diameter sensory neurons

Cell and AP Properties

IB4-negative cells

IB4-positive cells

 

Control

GRO/KC

Control

GRO/KC

Number of Cells

26

22

25

18

Diameter, μm

20.0 ± 0.8

21.2 ± 0.7

22.8 ± 0.5

20.2 ± 0.9*

Cell Capacitance, pF

17.6 ± 1.3

20.0 ± 1.2

19.0 ± 1.3

16.8 ± 1.7*

Resting Potential, mV

-63.0 ± 1.6

-56.0 ± 1.4**

-64.6 ± 1.4

-55.4 ± 1.9 **

Input Resistance, MΩ

1244.0 ± 144.3

986.1 ± 99.4

1062.0 ± 64.6

1427.4 ± 92.3**

AP Rheobase, pA

60.4 ± 8.4

21.1 ± 2.1**

76.2 ± 14.2

51.1 ± 11.2*

AP Threshold, mV

-19.7 ± 0.9

-28.6 ± 1.4**

-16.6 ± 0.6

-25.4 ± 1.3**

AP Rising Rate, mV/ms

106.8 ± 9.6

112.5 ± 10.7

103.4 ± 8.8

102.4 ± 12.0

AP Falling Rate, mV/ms

49.7 ± 4.8

61.9 ± 7.3

43.4 ± 3.8

39.9 ± 5.0**

AP Amplitude, mV

64.8 ± 1.7

74.8 ± 1.8**

63.1 ± 1.9

71.1 ± 1.5 **

AP Duration, ms

6.6 ± 0.4

7.3 ± 0.5

8.3 ± 0.5

14.0 ± 1.0**

AHP Amplitude, mV

3.5 ± 0.5

5.9 ± 0.6 **

3.0 ± 0.8

4.5 ± 0.7

AHP Duration, ms

10.8 ± 2.4

14.7 ± 0.8*

7.7 ± 1.7

9.9 ± 1.4

  1. Values are mean ± SEM. AP, action potential; AHP, afterhyperpolarization. * P < 0.05, ** P < 0.01, comparing GRO/KC treated vs. Control cells with the same IB4 status (Student's t-test or Mann-Whitney test). Rheobase is defined as the smallest depolarizing current to elicit at least one action potential within 80 msec. Action potential parameters were measured on this first elicited action potential.