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Figure 9 | Molecular Pain

Figure 9

From: Differential regulation of morphine antinociceptive effects by endogenous enkephalinergic system in the forebrain of mice

Figure 9

Detection and measurement of μ -opioid receptors by immunostaining and Western blotting. (A) Immuoreactive μ-opioid-receptors were stained in the S1 and ACC of WT and KO mice. Micrographs of μ-opioid-receptors (100× magnification) are shown in the upper panel. Micrographs at higher magnification are shown in the lower panel. Locations that were immunopositive for μ-opioid-receptors are indicated by square boxes in the left panel. (B) Western blot data provided a quantitative measurement of μ-opioid receptors in the cortical region of WT and KO mice. The μ-opioid receptor band is located at 43 kDa (arrow heads). Non-specific binding was used as an internal control (56 kDa). (C) The relative quantities of μ-opioid receptor were measured by taking the ratio of specific and non-specific binding in the S1 and ACC of WT (n = 6) and KO (n = 7) mice. Data are presented as the mean ± SEM.

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