Figure 5
From: Direct activation of Transient Receptor Potential Vanilloid 1(TRPV1) by Diacylglycerol (DAG)
OAG-induced TRPV1 activation is PKC and DAG-lipase independent. (Aa) OAG-induced current was well generated in 1 μM chelerythrine, a PKC inhibitor in the internal pipette solution in the whole cell patch mode on the condition of 1 min (left trace) and 10 min (right trace) after rupturing cell. (Ab) OAG-induced current was also examined in 10 μM RHC80267, a DAG-lipase inhibitor in the same manner. (c) The summary bar graph was derived from the current peak amplitude in both conditions a PKC inhibitor and a DAG-lipase inhibitor at each 1 min and 10 min recording data. Each inhibitor was included in the patch pipette solution. (d) Co2+ uptake by OAG in TRPV1 expressing HEK 293 cells were observed even in the presence of 1 μM chelerythrine and 10 μM RHC80267 and was blocked by 10 μM CZP, similar to 1 μM capsaicin.