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Figure 1 | Molecular Pain

Figure 1

From: Expression of AMPA receptor subunits at synapses in laminae I–III of the rodent spinal dorsal horn

Figure 1

Immunostaining with the pan-AMPAr antibody and with subunit-specific antibodies in laminae I–III of the rat dorsal horn following antigen retrieval with pepsin. Each image shows a vertical strip taken through the central part of the dorsal horn stained with the pan-AMPAr antibody (pAMPA) or with one of the subunit-specific antibodies. As we have reported previously [60], lamina I is relatively thick in this region. Images of pan-AMPAr, GluR1 and GluR2 are taken from one section, while those for GluR3 and GluR4 are taken from another. GluR1 puncta are most numerous in laminae I and II, while those that are GluR2- or GluR3-immunoreactive are present in large numbers throughout laminae I–III. The GluR4 staining was obtained with a rabbit antibody against the C-terminal part of the protein (GluR4-C antibody), and is present at relatively few puncta in laminae I and II. However some clusters of GluR4 puncta that are orientated either transversely (arrow) or dorsoventrally (arrowhead) are visible in the superficial dorsal horn. Each image was obtained from a projection of 5 optical sections at 0.3 μm z-spacing. Approximate locations of laminar boundaries are shown. Scale bar = 20 μm.

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