Figure 3
From: Expression of AMPA receptor subunits at synapses in laminae I–III of the rodent spinal dorsal horn
Staining with GluR1-4 and pan-AMPAr antibodies. Confocal images that show immunoreactivity for GluR1, GluR3 and pan-AMPAr (pAMPA) in lamina I (a-d), and GluR2, GluR4 and pan-AMPAr in lamina III (e-h). In each case a merged image is shown (d,h). Note that all puncta labelled with the GluR1-4 antibodies are also labelled with the pan-AMPAr antibody. a-d: Although most of the puncta that are strongly labelled with the GluR1 or GluR3 antibody are weakly labelled or negative with the other one, some can be seen to contain immunostaining for both subunits (2 shown with arrows). e-f: All of the puncta that are GluR4-positive are also labelled with the GluR2 and pan-AMPAr antibodies. The images are projections of 2 optical sections at 0.35 μm z-spacing. Scale bar = 10 μm.