Quantitative Real-Time PCR detection of TRPV1–4 gene expression in human leukocytes from healthy and hyposensitive subjects

Table 1 Details of primers and amplicons for the 4 HKGs and the 4 GOIs analysed in this study.

Gene	Acc. Number	Forward Primer Sequence [5'→3']	Position in cDNA	Reverse Primer Sequence [5'→3']	Position in cDNA	Amplicon Length	E%	R²
Act-B*	NM_001101	CTGGAACGGTGAAGGTGACA	6^th	AAGGGACTTCCTGTAACAATGCA	6^th	140 bp	96.6	0.99
GAPDH*	NM_002046	TGCACCACCAACTGCTTAGC	7^th	GGCATGGACTGTGGTCATGAG	7^th/8^th	87 bp	91.8	0.998
HPRT1	NM_000194	AGATGGTCAAGGTCGCAAG	6^th	GTATTCATTATAGTCAAGGGCATATC	8^th	128 bp	104.2	0.995
hCyPB	M60857	CCAACGCAGGCAAAGACACCAA	4^th	GCTCTCCACCTTCCGCACCA	5^th	131 bp	91.2	0.995
TRPV1	NM_080704	GGCTGTCTTCATCATCCTGCTGCT	12^th	GTTCTTGCTCTCCTGTGCGATCTTGT	12^th	117 bp	94.2	0.99
TRPV2	NM_016113	CTTCCTTTTCGGCTTCGCTGTAG	10^th	GCACTGACTCTGTGGCATTGG	11^th	95 bp	89.7	0.99
TRPV3	NM_145068	TCCTCACCTTTGTTCTCCTCCT	14^th	CGCAAACACAGTCGGAAATCAT	15^th	211 bp	103.6	0.995
TRPV4	NM_021625^†NM_147204^‡	CTACGCTTCAGCCCTGGTCTC	11^th/12^th†	GCAGTTGGTCTGGTCCTCATTG	13^th ^†	76 bp	103.7	0.99

* refers to RT-PCR primers taken from [21]. † refers to TRPV4 isoform a and ‡ to TRPV4 isoform b.

ISSN: 1744-8069