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Figure 1 | Molecular Pain

Figure 1

From: Sumatriptan inhibits synaptic transmission in the rat midbrain periaqueductal grey

Figure 1

5-HT inhibits evoked IPSCs in PAG neurons. (A) Time course of evoked IPSC amplitude (eIPSC Ampl) during application of 5-HT (10 μM), baclofen (10 μM) and CGP55845 (CGP, 1 μM). (B) Averaged evoked IPSCs before (control) and during application of 5-HT and baclofen. (C) Averaged evoked IPSCs in response to identical paired stimuli (inter-stimulus interval = 70 ms) for the traces in B, with IPSC1 normalized to demonstrate relative facilitation of IPSC2 during superfusion of 5-HT. (D) Concentration-response curve of the reduction in evoked IPSC (eIPSC) amplitude produced by 5-HT, expressed as a percentage of the pre-5-HT level, with a logistic function fitted to determine the IC50. (E) Bar chart showing the amplitude of the first evoked IPSC (eIPSC1) and the ratio of evoked IPSC2/IPSC1 (eIPSC2:1) in the presence of 5-HT (10 μM), fluoxetine (30 μM) and baclofen (10 μM), expressed as a percentage of the pre-drug level. In (D) *, ** and ## denote p < 0.05, 0.01 and 0.0001. Traces in (A – C) are from the same neuron.

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