Figure 2

mRNA for 5-HT1A, B, D and F receptors is expressed in rat PAG. A representative agarose gel illustrating amplified fragments of mRNA for each of four 5-HT1 receptor subtypes in PAG slices of a male and a female rat. The lane marked M is the molecular weight ladder, the arrows indicate 200 D (lower) and 500 D (upper), respectively. The first lane for each receptor is from a male rat, the second from a female, the third is the no-template control for reagent contamination. 5-HT1A is Lanes 1–3, 5-HT1B is Lanes 4–6, 5-HT1D is Lanes 7–9, 5-HT1F is lanes 10–12. The primers used to amplify the mRNA are listed in Table 1.