Figure 3

5-HT1A, 5-HT1B and 5-HT1D receptor activation inhibits evoked IPSCs in PAG neurons. Averaged evoked IPSCs are shown prior to (control) and during (agonist) addition of (A) the 5-HT1A agonist 8-OH-DPAT (3 μM), (B) the 5-HT1B agonist CP93129 (3 μM), (C) the 5-HT1D agonist L694247 (3 μM) and (D) the 5-HT1F agonist LY344864 (3 μM). (E) Bar chart showing the percentage inhibition of evoked IPSC amplitude (eIPSC Ampl) produced by 5-HT (1 μM), 8-OH-DPAT (3 μM), CP93129 (3 μM) L694247 (3 μM) and LY344864 (1 – 3 μM) expressed as a percentage of the pre-drug level. In (E), *, **, # and ## denote p < 0.05, 0.01, 0.001 and 0.0001. Traces in (A – D) are from different neurons, bars are 200 pA and 10 ms.