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Table 1 The sequence of the primers used to amplify 5-HT1 receptor mRNA from PAG tissue.

From: Sumatriptan inhibits synaptic transmission in the rat midbrain periaqueductal grey

Receptor

Product size (bp)

Primers

5-HT1A

113

5'-TCTGTTGCTGGGTACTCTCA-3'

  

5'-AGGAGCCGATGAGATAGTTG-3'

5-HT1B

151

5'-ATGGAGGAGCAGGGTATTCA-3'

  

5'-AAGCAACCAGCAGGACTTTC-3'

5-HT1D

171

5'-ATCTCAGATTTCTTACACCATCTACTC-3'

  

5'-CGCAGAGCCCGTGATAAG-3'

5-HT1F

108

5'-ACCACCCAGCCAACTATTTA-3'

  

5'-TAATCCAACTCTCGCTCACA-3'

  1. For each receptor the forward and reverse primers are shown, respectively. bp is base pairs.
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Molecular Pain

ISSN: 1744-8069

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