Figure 3

Quantitative analysis of sympathetic and sensory fibers in the upper dermis of saline-treated rats and in CFA-treated rats at 1, 2, 3 and 4 weeks post-injection. For sympathetic fibers (A), the number of fibers in the upper dermis was counted, whether for the sensory fibers the total length of fibers per mm² was calculated. For details regarding quantification, see Material and Methods. In A, note that the average number of sympathetic fibers in the upper dermis was higher in the CFA group than in the saline group at 2, 3 and 4 weeks post-injection, although that increase reached significance levels at the 4 week time point only. One-way ANOVA followed by the Dunnett post hoc test. In B, note that there was a significant difference in the density of the sensory peptidergic (CGRP-IR) innervation when comparing saline and CFA-treated groups, starting at 2 weeks-post-CFA injection. Statistical analyses were performed by one-way ANOVA followed by the Dunnett post hoc test. Significance was established at P < 0.05.