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Figure 1 | Molecular Pain

Figure 1

From: Long-term actions of interleukin-1β on delay and tonic firing neurons in rat superficial dorsal horn and their relevance to central sensitization

Figure 1

A. Time course of media exchanges in spinal cord organotypic cultures, establishment of defined medium and time course of IL-1β application. Nerve Growth Factor (NGF) was 20 ng/ml. Serum was progressively removed by successive dilutions during exchanges of medium. Pen/Strep/Amp = 5 units/mL penicillin G, 5 units/mL streptomycin, and 12.5 ng/mL amphotericin B, Ara-C/U/5 FDU = uridine, cytosine-β-D-arabino-furanoside (AraC), and 5-fluorodeoxyuridine (all at 10 μM). B. Comparison of time course of present IL-1β studies with previous studies of sciatic chronic constriction injury (CCI) [21]. Time scale in days refers to age of experimental animals. C. Photomicrograph obtained from an acutely isolated spinal cord slice. The central canal appears as the light area just to the right of the upper centre of the picture. The ventral surface of the cord is to the left D. Photomicrograph obtained from an organotypic culture, Sections in C and D both immuno-reacted for the microglial marker Iba-1. Calibration bar in both plates is 50 μm.

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Molecular Pain

ISSN: 1744-8069

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