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Figure 2 | Molecular Pain

Figure 2

From: Long-term actions of interleukin-1β on delay and tonic firing neurons in rat superficial dorsal horn and their relevance to central sensitization

Figure 2

Effects of 6–8 d exposure of the dorsal hornregion of organotypic cultures to 100 pM IL-1β A . Typical time courses of changes in Fluo 4-AM fluorescence, indicating changes in free intracellular [Ca2+] in response to raising [K+] in superfusate from 2.5 to 20 mM. Traces are recordings from somata of cells marked as regions of interest for the analysis software. B and C. Quantification of Fluo-4 fluorescence changes in response to 20, 35 and 50 mM K+ from 26 control and 26 IL-1β treated neurons. Changes in amplitude in response to 50 mM K+ are increased in cytokine-treated slices (p = 0.02). For area under curve (AUC) significant increases are seen for both 35 mM (p < 0.01) and 50 mM K+ (p < 0.001).

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