Skip to main content
Figure 5 | Molecular Pain

Figure 5

From: Gene transfer of GLT-1, a glial glutamate transporter, into the spinal cord by recombinant adenovirus attenuates inflammatory and neuropathic pain in rats

Figure 5

Effect of spinal GLT-1 gene transfer on the spinal GLT-1 expression in the total and membrane fraction following pSNL. Seven days after Ad-EGFP or Ad-GLT-1 was infused intraspinally, pSNL or sham surgery was performed. Seven days later (14 days after the intraspinal infusion), the GLT-1 expression in the total (A, B) and membrane (C, D) fractions obtained from ipsilateral spinal cord was assessed by Western blot. A, C) Representative immunoblots show GLT-1 and actin immunoreactivity in total and membrane fractions. No actin band was observed in the membrane fraction. B, D) Quantification of GLT-1 immunoreactivity in the total and membrane fractions. The signals were calculated by summing the monomer and multimer GLT-1 immunoreactivities. Total GLT-1 levels were normalized against the corresponding actin levels. The data present the means ± S.E.M. and are expressed as a percentage of the control (intraspinal infusion of Ad-EGFP and sham surgery) for each fraction. *P < 0.05, n = 3–4.

Back to article page

Molecular Pain

ISSN: 1744-8069

Contact us