Figure 3
Expression and distribution of total GluR1 and GluR2 in spinal cord dorsal horn. A and B: Top: representative Western blots showing GluR1 protein (A) and GluR2 protein (B) in total soluble fraction derived from the ipsilateral and contralateral L4–5 dorsal horns of naïve rats (n = 4) and the rats at 2 and 24 h post-saline (S; n = 4/time point) or post-CFA injection (C; n = 4/time point). Bottom: statistical summary of the densitometric analysis expressed relative to the corresponding loading control (β-actin). C: Representative photographs showing the distribution of GluR1 immunoreactive cells (arrow heads) in lamina V and of GluR2 immunoreactive cells (arrows) in laminae II-III. Scale bar: 20 μm. D and E: Representative photographs showing distribution of GluR1 (D) and GluR2 (E) immunoreactivity in the ipsilateral and contralateral L4 dorsal horns at 2 and 24 h post-CFA injection. Scale bar: 200 μm. F: Statistical summary of the optical density of GluR1 and GluR2 immunoreactivity in the ipsilateral and contralateral L4–5 dorsal horns at 2 and 24 h post-saline (n = 3/time point) or post-CFA (n = 3/time point) injection. N: naïve (n = 3). Data are presented as mean ± SEM.