Changes in blood glucose level, body weight and thermal pain sensitivity in a double transgenic mice (TCR-SFE/Ins-HAD2) model of diabetes. A. By six weeks of age, double transgenic mice became diabetic (blood glucose levels >600 mg/dl) and remained high (filled circles, n = 9, p < 0.001) throughout the course of the experiment as compared to non-diabetic control (Ins-HAD2) mice (open circles, n = 6). B. Body weights of TCR-SFE/Ins-HAD2 mice remained constant (filled circles, n = 9), while control non-diabetic mice (Ins-HAD2) gained weight steadily (open circles, n = 6, p < 0.001) C. PWL measured from the age of 6 weeks to 18–19 weeks revealed three phenotypes as compared to control non-diabetic mice (Ins-HAD2) (open circles, n = 19). The first group exhibited an initial phase of hyperalgesia (open squares, n = 12, p < 0.001) followed by a phase of hypoalgesia (n = 8, p < 0.05). The second group showed no hyperalgesic phase, but became hypoalgesic (filled squares, n = 8, p < 0.001). The third group became hyperalgesic and remained hyperalgesic throughout the course of the study (filled circles, n = 12. Asterisks (*, **) represent p < 0.05 and p < 0.001, respectively.