Figure 9

ASIC3-containing afferents in inflamed muscle. Red labeling shows secondary antibodies conjugated with Alexa Fluor-594, green with Alexa Fluor-488. A, B, C, ASIC3 immunoreactive nerves were found surrounding the vessels and co-localized with PGP9.5. D, E, F, G, In nerve bundles, ASIC3 immunoreactivity was co-localized with PGP9.5 (yellow spots in G). H, I, J, K, ASIC3 and PGP9.5 had no immunoreactivity in areas surrounding granuloma. L, M, N, O, ASIC3 and PGP9.5 immunoreactive nerves were found on a vessel with vasculitis. A, D, H, L, shows the contrast pictures. Yellow or black arrows indicate the co-localization of ASIC3 and PGP9.5; the red arrow indicates positive staining for PGP9.5 but not ASIC3. The arrowhead shows a well-formed granuloma.