Figure 4
From: Peripheral nerve injury sensitizes neonatal dorsal horn neurons to tumor necrosis factor-α
TNFα reduces NMDAR function in neonatal SDH neurons following nerve injury during early postnatal development. (A) Representative traces of AMPAR-mediated (black) and NMDAR-mediated (gray) EPSCs recorded from immature SDH neurons (following SNI at P6) after incubation with vehicle solution (left) or TNFα (right). Each EPSC represents the average of 10 responses evoked from a holding potential of +50 mV via focal stimulation within the SDH (see Methods). (B) TNFα significantly increased the ratio of AMPAR-to-NMDAR current amplitudes compared to vehicle controls (*p = 0.022; Mann-Whitney test). (C) Incubation with TNFα did not change the mean amplitude of evoked AMPAR currents (left) in SDH neurons under neuropathic conditions, but did significantly decrease NMDAR current amplitudes (right; *p = 0.024; Mann-Whitney test).