Figure 4

NF-κB is required for COMT downregulation. (A) Cotransfection of pCMV-SPORT-M-p65 or pCMV-SPORT-M-IKKβ inhibits P2-COMT/Luc reporter activity in H4 cells. Data are Means ± SEM. **P < 0.01 different from P2-COMT/Luc alone. Western blot analysis was performed with protein extracts from H4 cells treated with TNFα (20 ng/ml) for the indicated timepoints. Expression of IκBα was evaluated in cytoplasmic fraction of H4 cells (B), whereas p65 and p50 protein levels were tested in nuclear extracts (C).