Table 1 Plasmids and enzymes used for synthesis of the cDNA probes
From: Transforming growth factor-β1 impairs neuropathic pain through pleiotropic effects
Plasmid | Vector | Insert | Antisense probe | Sense probe | GenBank Reference |
|---|---|---|---|---|---|
Topo+TGFβRI | BluntIItopo | 597 pb | Pst1/Sp6 | HindIII/T7 | NM-009370 |
Topo+TGFβRII | BluntIItopo | 592 pb | BamHI/T7 | XbaI/Sp6 | D32072 |
pBSKS+ TGF-β1 | pBSKS+ | 1,173 pb | EcorI/T3 | XhoI/T7 | BC013738 |