Figure 6

HC-030031 inhibits mechanically-evoked action potentials in wild type mice but not in TRPA1-deficient mice. A) C fibers from Trpa1^+/+ mice were tested with sustained (10 sec) mechanical force in increasing intensities from 5 to 200 mN in the presence of vehicle (solid squares) or HC-030031 (100 μM; open circles; p = 0.020, 2-way repeated measures ANOVA). B) C fibers from Trpa1^-/- mice were tested with sustained (10 sec) mechanical force in increasing intensities from 5 to 150 mN in the presence of vehicle (solid squares) or HC-030031 (100 μM; open circles; p > 0.05, 2-way repeated measures ANOVA). C) Adaptation rate of action potential firing in C fibers from Trpa1^+/+ mice during a 10 sec, 150 mN mechanical force in the presence of vehicle (black bars) or HC-030031 (100 μM; grey bars). HC-030031 significantly inhibited the firing rate after the first second (p = 0.024, 2-way ANOVA). D) Adaptation rate of action potential firing in C fibers from Trpa1^-/- mice during a 10 sec, 150 mN mechanical force in the presence of vehicle (black bars) or HC-030031 (100 μM; grey bars). HC-030031 had no effect on the adaptation rate in C fibers from Trpa1^-/- mice (p > 0.05, 2-way ANOVA).