Figure 2
E 2 -induced inhibition of I Gly is independent of intracellular signaling pathways and classical estrogen receptors (ERs). (A) Sample traces illustrating the inhibitory effects of E2 on the peak I Gly under the conditions of intracellular application 15 mM BAPTA (A1), 5 μM staurosporine (A2), 0.5 mM GTP-γ-S (A3) and 0.5 mM GTP-β-S (A4), respectively. A5, Effect of E2 on I Gly after incubation of neurons with tamoxifen for 2 h. A6, Effect of 17-α-E2 on I Gly. (B) Pooled data summarizing the effect of E2 on I Gly under various conditions shown in A. Each column represents the average values from 4–6 neurons, ***P < 0.001, Paired Student's t-test, compared with control without adding E2 or 17-α-E2 (dashed line). NS indicates no significant difference in this and the following figures.