Figure 5
Developmental regulation of E 2 inhibition on I Gly . Hippocampal and spinal cord neurons in culture were used for electrophysiological recording following 6–8, 13–15 and 20–23 days of in vitro (DIV) differentiation to examine the developmental dependence of E2 inhibition on I Gly. Each column represents the average value of 5–8 neurons. * P < 0.05 and *** P < 0.001 (Student's paired t test, n = 8), compared to control without adding E2; # P < 0.05 (one-way ANOVA, n = 8), comparing DIV 20–23 with either DIV 6–8 or DIV 13–15.