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Figure 6 | Molecular Pain

Figure 6

From: Glycine receptor in rat hippocampal and spinal cord neurons as a molecular target for rapid actions of 17-β-estradiol

Figure 6

Inhibitory effect of E 2 on GlyR-mediated tonic current in HIP slices. (A) Left, Whole-cell voltage-clamp recording showing the current in the presence of 0.3 μM TTX, 10 μM bicuculline, 3 μM CNQX, 10 μM APV, 20 μM glycine and 0.5 mM sarcosine. Application of strychnine (2 μM) decreased the membrane current noise and revealed the tonically activated GlyR current. In the presence of E2 (10 μM), the amplitude of GlyR-mediated tonic current was significantly reduced. Right (a1–a3), Gaussian fit to all-point histograms of 30 s traces at the time point a1, a2 and a3 (A, inset). The differences among the Gaussian means are marked by the dotted lines. (B) The normalized GlyR-mediated tonic current in the absence or presence of E2 (n = 8). *P < 0.05, Paired Student's t-test, compared with the control without E2 treatment.

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