Figure 1
From: Antidepressants inhibit P2X4 receptor function: a possible involvement in neuropathic pain relief
Effect of antidepressants on the ATP-evoked [Ca2+ ] i response via rat P2X 4 receptors. Three rounds of ATP stimulation (30 μM, 20 sec) induced [Ca2+]i response in a reproducible fashion (a). Effect of pretreatment of cells with EGTA (500 μM, 10 min) (b) or with paroxetine (10 μM, 10 min) (c) on [Ca2+]i response evoked by the second ATP stimulation. Traces indicate 340/380 fura-2 emission ratios averaged from (a) 48 cells, (b) 66 cells and (c) 18 cells obtained from each representative experiment. Effect of pretreatment of cells with TNP-ATP, antidepressants and anticonvulsants (10 μM, 10 min) on the ATP-evoked [Ca2+]i response via rat P2X4 receptors (d). Paroxetine dose-dependently inhibited the [Ca2+]i response via rat P2X4 receptors with an IC50 value of 2.45 μM for 30 μM ATP stimulation (e). ATP dose-response curve was generated in the presence of increasing concentrations of paroxetine (f). Data are means ± SEM of at least 10 cells (d, e and f).