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Figure 6 | Molecular Pain

Figure 6

From: Activation of cytosolic phospholipase A2 in dorsal root ganglion neurons by Ca2+/calmodulin-dependent protein kinase II after peripheral nerve injury

Figure 6

cPLA 2 and CaMKII are activated via stimulation of P2X 3 and P2X 2+3 receptors in primary DRG neurons. (A-C) Western blot analyses of p-cPLA2 and p-CaMKII proteins after applying ATP or α,β-methylene ATP (αβmeATP) to rat primary DRG neurons. The primary DRG culture was incubated with ATP or αβmeATP. KN-92, KN-93, A-317491 or cadmium was added to the neurons 10 min before the application of ATP or αβmeATP. The total cPLA2 and CaMKII proteins loaded on each lane were also detected as loading controls. The bar graphs show the relative values of p-cPLA2 and p-CaMKII proteins induction with respect to controls, after normalizing for the cPLA2 and CaMKII protein levels, respectively. *p < 0.05 compared with the control group. #p < 0.05 compared with the ATP- or αβmeATP-stimulated group. (D) Increase in the levels of p-cPLA2 and p-CaMKII immunofluorescence and translocation of these proteins to the plasma membrane in neurons 5 min after the application of 10 μM BayK8644 compared with controls. Scale bar, 20 μm.

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