Figure 4

ATP receptors relation to PDGF-BB-induced allodynia. (A) Total RNA extracted from the L5 spinal cord on day 3 after PDGF-BB (10 pmol) administration was subjected to quantitative analysis of P2X₄, P2X₇, and P2Y₁₂ receptors mRNA expression by real-time RT-PCR. Data are means ± SEM of the fold change over vehicle control (n = 5). *P < 0.01 vs vehicle by Student's t-test. (B) The paw withdrawal thresholds before (pre) and 7 days (day7) after intrathecal PDGF-BB (10 pmol) administration (n = 8). Then TNP-ATP (30 nmol) and vehicle (PBS) was intrathecally administered on day 7 and the changes in the paw withdrawal thresholds were measured (n = 4). Data are means ± SEM of the thresholds. ^### P < 0.001 vs pre, *P < 0.05 vs vehicle group by Student's t-test.