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Figure 1 | Molecular Pain

Figure 1

From: Inhibitory coupling between inhibitory interneurons in the spinal cord dorsal horn

Figure 1

Characterization of the GAD65-EGFP+ neurons. A. Photomicrograph of a lumbar transverse section of the spinal cord showing the distribution of EGFP expression. Scale bar = 200 μm. B. immunostaining for parvalbumin (red) and GFP (green) shows some co-localization in lamina II (yellow). Scale bar = 50 μm. C. Examples of EGFP+ neurons, reconstructed from neurobiotin/Lucifer Yellow filled cells. a-b: islet cells, c: vertical cell, d-e: unclassified cells. All cells are shown in the parasagittal plane. The majority of the dye filled cells were islet cells (9/15), one cell was vertical and the rest (5/15) could not be classified. D. Firing patterns in response to 500 ms-long depolarizing and hyperpolarizing current steps from a holding potential of -65 mV. Left: The response of a tonic firing neuron to current steps of -30 pA and +40 pA. Middle: A neuron that fired one or few spikes at the onset of the depolarization. Responses to current steps of -30 pA and +40 pA. The Inset shows the average instantaneous firing frequency (f) plotted against stimulus intensity (I). The f-I curve for this neuron being linear, it was classified as tonic firing according to previously established criteria [20]. Right: Response of a single spiking neuron to -30 pA and +90 pA current steps.

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