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Figure 2 | Molecular Pain

Figure 2

From: Inhibitory coupling between inhibitory interneurons in the spinal cord dorsal horn

Figure 2

Lamina II inhibitory neurons receive both GABA A R- and glycine receptor-mediated inhibitory inputs. IPSCs evoked by focal electrical stimulation while recording at a holding potential (HP) of 0 mV using CsMeSO3-filled micropipettes (low [Cl-]i). The traces represent averages of 10 consecutive responses. These IPSCs displayed complex kinetics. Administration of strychnine (0.5 μM; Left) blocked the fast decaying component, indicating that it was mediated by activation of glycine receptors, while administration of SR95531 (gabazine, 10 μM; Right) blocked the slow decaying component, indicating that is was mediated by activation of GABAA receptors. The relative contribution of the two components varied from cell to cell: the fast component comprised 78% (range 54%–94%) of the peak, while the slow component 22% (range 6%–46%) Inset: IPSCs evoked by focal stimulation while recording at a holding potential of -70 mV using CsCl-filled micropipettes (high [Cl-]i) to record inward currents (Scale bars = 25 pA, 50 ms). Recordings were performed in the presence of strychnine and addition of SR95531 abolished all remaining inward currents indicating that the evoked responses were entirely mediated by GABAA and glycine receptors.

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