Skip to main content

Advertisement

Table 1 Quantitative PCR measurements of selected voltage-gated K channels

From: NF-κB mediated enhancement of potassium currents by the chemokine CXCL1/growth related oncogene in small diameter rat sensory neurons

Channel Type* Rat gene symbol Rat Gene ID Abundance in control cells relative to
Kv 1.1
Fold-change w/GRO/KC
Kv 1.1 DR Kcna1 [GenBank: 24520] 1.00 ± 0.17 1.18 ± 0.12
Kv 1.2 DR Kcna2 [GenBank: 25468] 0.25 ± 0.04 1.24 ± 0.19
Kv 1.3 DR Kcna3 [GenBank: 29731] 0.06 ± 0.04 0.81 ± 0.23
Kv 1.4 A-type Kcna4 [GenBank: 25469] 0.16 ± 0.11 0.82 ± 0.22
Kv 3.1 DR Kcnc1 [GenBank: 25327] 0.02 ± 0.00 1.14 ± 0.20
Kv 3.4 A-type Kcnc4 [GenBank: 684516] 0.05 ± 0.00 1.11 ± 0.09
Kv 4.3 A-type Kcnd3 [GenBank: 65195] 0.06 ± 0.01 0.89 ± 0.05
MinK-2 accessory Kcne3 [GenBank: 63883] 0.02 ± 0.00 0.98 ± 0.15
  1. N = 4 cultures from 4 animals, each of which provided a pair of RNA samples (control cells and GRO/KC treated cells). All expression values were normalized to that of the housekeeping gene HPRT from the same sample; fold changes (GRO/KC/control) were calculated for each individual pair of matched samples. For comparison of relative abundance of channel types in control cells, expression data were normalized to the most abundant channel observed, Kv1.1. None of the fold-change values after overnight GRO/KC incubation was significantly different from 1.
  2. *, channel type as given by reference [26], "DR" = delayed rectifier; however, K channel properties may also depend on accessory proteins and heteromer composition.