Figure 5

Effects of EphB2-Fc and targeted mutation of EphB1 receptor on the LTP of synapses between C-fibers and DH neurons in mice in vivo. LTP training protocol in A-D was the same as that in Fig. 2A–C: 100 Hz, 5 × threshold currents, 0.5 ms, 100 pulses, 4 trains of 1-s duration at 10-s intervals. Examples of the C-fiber-evoked field potentials given in each figure were recorded before (a) and after (b) tetanic stimulation (indicated by the arrow in each figure) alone (A, the same figures and data used as control as those in Fig. 2A) or combined with pre-treatment of EphB2-Fc (B) and IgG-Fc (C), respectively. The nonparametric Wilcoxon signed-rank test and the Kruskal-Wallis test were used to test thefield potentials within and between the groups, respectively (p < 0.01 in A and C; p > 0.05 in B and D; p < 0.01 between A/B, B/C, A/D and C/D; p > 0.05 between A/C and B/D).