Ionic currents of TRPA1, but not TRPM8, exhibited shifts in reversal potential. Outside-out patches were formed from HeLa cells expressing rat TRPA1 or human TRPM8 plus GFP. NMDG+ was the sole external cation and Na+ was the major internal cation. Membrane potential was held at -80 mV, and a voltage ramp from -140 to 0 mV (500 ms duration) was applied every 3 s immediately. A, current traces from a representative TRPA1-containing patch during 60 s application of AITC (100 μM). To illustrate shifts in Erev, only currents between -6 to 8 pA were plotted. Inset shows the AITC-evoked currents were almost completely blocked by 10 μM RR. B, currents from a representative TRPM8-containing patch during 60 s application of menthol (500 μM). Note the shifts in Erev for TRPA1, but no shift for TRPM8.