Time-dependent changes in ion permeability occurred in TRPA1 but not in TRPM8. A, Erev values were determined (from Fig. 3 experiments) and plotted as a function of time after application of AITC or menthol. B, permeability ratios (PNMDG/PNa) before and 60 s after agonist addition. Student's t-test was used with p < 0.05 as the criterion for significance (indicated by *). C, changes in Erev and relative currents at 0 mV (from representative recordings) were plotted as a function of time after application of AITC. Outward currents were measured at 0 mV and normalized against the current obtained after 60 s addition of AITC.