Thickness of mouse paws after peripheral inflammation. The wild-type CD1 mice (8–12 week-old) were injected in the right hind paws with 25 μl of capsaicin (100 μg/ml in saline containing 10% ethanol and 0.5% Tween 80, A), carrageenan (20 mg/ml, B), CFA (50% in saline, C), or saline (D). At 4, 24, and 72 hours after injection, the mice were killed and the thickness of injected (ipsilateral) and uninjected (contralateral) paws was measured. All data are presented as mean ± SEM of total tested mice (n = 6–12). Comparison of inflammatory agent-injected and saline-injected animals (*) or between contralateral and ipsilateral paws of agent-injected animals (#) was by t test. *# p < 0.05, ** ##p < 0.01, ***###p < 0.001.