Number of TDAG8-expressing neurons increases in both IB
-positive and -negative neurons after CFA-induced inflammation. After CFA-injection, lumbar 4–5 DRG ipsilateral and contralateral to injected paws were sectioned and hybridized with dig-labeled anti-sense mTDAG8 probes, followed by staining with antibodies against peripherin (PERI, red fluorescence) and IB4-FITC conjugates (IB4, green fluorescence). (A) Phase-contrast fields (a, d) are neurons labeled with cRNA probes. Fluorescence images (b, e) show neurons labeled with red (PERI only), green (IB4 only), and yellow (PERI and IB4). Phase-contrast images and fluorescence images were combined to obtain merged images (c, f). Arrows indicate the peripherin-positive neurons labeled with antisense probes. Arrowheads are the PERI and IB4 co-expressing neurons labeled with antisense probes. The scale bar is 50 μm. (B) The histogram shows the percentage of total PERI neurons that expressed TDAG8 in IB4-positive or -negative subpopulations.