Figure 2

Involvement of purinergic and NMDA receptors in ATP-induced nNOSNT-YFP translocation in PC12N cells. After a 30-min incubation of NGF-differentiated PC12N cells without or with 100 μM NMDA, 10 μM forskolin (FK), and 100 μM ATP in the absence and presence of 250 μM suramin, 100 μM PPADS, 100 μM RB-2, 100 μM MK-801 or 100 μM APV, the translocation of nNOSNT-YFP was observed by confocal microscopy and quantified as described in the legend for Figure 1B. Data are presented as the mean ± SEM (n = 4). *p < 0.05 compared with vehicle; ^# p < 0.05 compared with NMDA, FK, and ATP.