Figure 3

Effect of P2X and P2Y receptor agonists on nNOSNT-YFP translocation in PC12N cells. A. Stimulation of the translocation of nNOSNT-YFP by P2X and P2Y receptor agonists. After a 30-min incubation of NGF-differentiated PC12N cells without and with 100 μM 2MeSATP, UTP or ATP in the presence of 10 μM forskolin (FK) and 100 μM NMDA, the translocation of nNOSNT-YFP was determined by confocal microscopy as described in the legend for Figure 1B. Data are presented as the mean ± SEM (n = 4). *p < 0.05 compared with vehicle; ^# p < 0.05 compared with NMDA, FK, and ATP. B. Concentration dependency of ATP and its analogs for the translocation of nNOSNT-YFP in PC12N cells. NGF-differentiated PC12N cells were incubated for 30 min with the indicated concentrations of ATP (◇), 2-MeSATP (■), UTP (▲) or UTP + 2-MeSATP (○) in the presence of 10 μM FK and 100 μM NMDA. Data are presented as the mean ± SEM (n = 4). *p < 0.05 ATP vs. 2-MeSATP; ^# p < 0.05 ATP vs. UTP.