Figure 5
Involvement of cAMP in ATP-induced nNOSNT-YFP translocation. A. Effect of ATP on cAMP production in NGF-differentiated PC12 cells. The cells (1 × 104 cells/well) in 24-well plates were incubated for 15 min without or with 100 μM NMDA, 100 μM ATP or 1 nM PACAP in HBS containing 0.5 mM iso-butyl-1-methylxanthine. The cAMP content in the cells was measured by using the cAMP assay kit as described in "Methods." Data are presented as the mean ± SEM (n = 4–7). **p < 0.01 compared with vehicle. B. Effect of permeable cAMP and cGMP analogues on nNOSNT-YFP translocation. After a 30-min incubation of NGF-differentiated PC12N cells with vehicle, 500 μM 8-Br-cAMP or 500 μM 8-Br-cGMP in the presence of 10 μM forskolin and 100 μM NMDA, the translocation of nNOSNT-YFP was quantified as described in the legend for Figure 1B. Data are presented as the mean ± SEM (n = 4). *p < 0.05 compared with vehicle.